E.coliMismatchUracilDNAGlycosylase(Mugprotein),alsoknownasG/Umismatch-specificDNAglycosylase,isan18kDaconstitutivelyexpressedproteinwhichbelongstotheTDG/mugDNAglycosylasefamily.IthasbeenproposedthattheMugproteinexcises3,N4-ethenocytosineandremovestheuracilbasefrommismatchesintheorderofU:G>U:A,althoughtheBIOLOGicalroleremainsunclear.UracilbasesinDNAcanarisefromdeaminationofcytosinegivingrisetoincreasedspontaneousmutations.TheenzymeUracil-N-GlycosylaseremovesuracilfromtheDNAleavinganAPsite.Itiscapableofhydrolyzingthecarbon-nitrogenbondbetweenthesugar-phosphatebackboneoftheDNAandthemispairedbase.Thecomplementarystrandguaninefunctionsinsubstraterecognition.RequiredforDNAdamagelesionrepairinstationary-phasecells.
| Source | E.coli |
| Names | G/UMismatch-SpecificDNAGlycosylase,Double-Strand-SpecificUracilGlycosylase,Mismatch-SpecificUracilDNA-Glycosylase,MUG,mug,ygjF |
| Accession# | P0A9H1 |
| Formulation | Suppliedasa0.2μMfilteredsolutionof20mMTris-HCl,2.5mMβ-ME,1mMPMSF,50%Glycerol,pH8.0 |
| Shipping | Theproductisshippedondryice/icepacks. |
| Storage | Storeat<-20°C,stablefor6monthsafterreceipt. Pleaseminimizefreeze-thawcycles. |
| Purity | Greaterthan95%asdeterminedbySEC-HPLCandreducingSDS-PAGE. |
| AminoAcidSequence | MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQAGFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELDQALVVRGRLEHHHHHH |
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