Westernblot | At0.25-0.5μg/mlwiththeappropriatesystemtodetectcalcineurinalphaincells |
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andtissues. |
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Immunohistochemistry | At0.5-1μg/mltodetectcalcineurinalphainformalinfixedandparaffinembedded |
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(Paraffin-embeddedSection) | tissues. |
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Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.
Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.
1.Giri,P.;Higuchi,S.;Kincaid,R.L.:Chromosomalmappingofthehumangenesforthecalmodulin-dependentproteinphosphatase(calcineurin)catalyticsubunit.Biochem.Biophys.Res.Commun.181:252-258,1991.
2.Sun,L.;Blair,H.C.;Peng,Y.;Zaidi,N.;Adebanjo,O.A.;Wu,X.B.;Wu,X.Y.;Iqbal,J.;Epstein,S.;Abe,E.;Moonga,B.S.;Zaidi,M.:Calcineurinregulatesboneformationbytheosteoblast.Proc.Nat.Acad.Sci.102:17130-17135,2005.
3.Wang,M.G.;Yi,H.;Guerini,D.;Klee,C.B.;McBride,O.W.:CalcineurinAalpha(PPP3CA),calcineurinAbeta(PPP3CB)andcalcineurinB(PPP3R1)arelocatedonhumanchromosomes4,10q21-q22and2p16-p15respectively.Cytogenet.CellGenet.72:236-241,1996.
