Everest Biotech/L-Lactate Assay Kit I(10ml) 200 Assays- Assay Solution ONLY/120001200A/(10ml)200 assays- ASSAY SOLUTION,Everest Biotech,,Everest Biotech,everestbiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Everest Biotech/L-Lactate Assay Kit I(10ml) 200 Assays- Assay Solution ONLY/120001200A/(10ml)200 assays- ASSAY SOLUTION

产品编号：120001200A

市场价：¥1670.24

场地：美国(厂家直采)

产品分类：试剂盒>其它试剂盒>其它检测试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$160.60

品牌： Everest Biotech

公司：Everest Biotech

公司分类：

商品介绍

SampleTyple	Plasma,Serum,Cellculturesupernatant,otherbodyfluid
Contents
Method	Colorimetricmethodat490nm
StandardCurve
Sensitivity	60µM-3000µM
ReactionTime	30minutes
Applications	ForBIOLOGicalresearch: L-Lactatemeasurementinbiologicalsamples Fordrug/pharmresearch: DruginfluenceonL-Lactatemetabolism
Storage	-80°C
Notes	Materialsneededbutnotsupplied Aplatereadercapableofmeasuringabsorbancebetween470-490nm AdjustablePipettesandarepeatpipettor Asourceofpurewater;glassdistilledwaterofHPLC-gradewaterissufficient 0.5MAceticAcid Clearflatbottom96-wellplatesifnotincludedinthekitpurchased
Shipping	Icepacks

ReagentPreparation

Note:Allreagentsarefrozen.SWerecommendyouspinsmallvialsbeforeopening.

1.L-LactateStandards
Thevialcontains1000µlof3mML-LactateStandard.Thestandardmustbeequilibratedtoroomtemperaturebeforeuse.1mlofthestandardisenoughformaking3standardcurvesifassayedinduplicate.Storeat-80?C.

2.L-LactateAssaySolution
Thesolutioncontainsenzymesthatarelightsensitive.Thesolutionmustbethawedonicebeforeuse.Besttoaliquottheamountneededanduseitalltopreventthawing/freezingcycles.Freezeandstorealiquotsat-80oC

Protocol

1.SamplePreparation

Serum/Plasma/otherbodyfluid/cellculturesupernatant
Serum,Plasma,otherbodyfluid,orcellculturesupernatantcanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereADIngsarewithinthestandardcurve.
YoursamplescanbedilutedwithdH2O.
Note:ifsamples(likehemolyzedserum/plasmaorcellculturemediumwhichcontainFBS)containhighleveloflactatedehydrogenasecapableofconvertinglactatetopyruvate,itisimportantforthesamplestobedeproteinated.

SolidSamples
Solidsamples,suchastissues,canbefirsthomogenizedandextractedwithethanol(80%)withatissues/Ethanolratioof1:8(1hrat4°C)followedbycentrifugationat10,000xg.Theclearsupernatantsthencanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereadingsarewithinthestandardcurve.YoursamplescanbedilutedwithdH2O.

AddingSamples
Add50μlofsamplestoeachwell.
Werecommendthatsamplesbeassayedinduplicate.

Note:
Ifpreparedsamplesarenotassayedthesameday,storethesamplesat-80?C.Ifsamplesneedtobedeproteinated,makesuretodeproteinizethesamplespriortostoringinthefreezer.Thedeproteinatedsampleswillbestableforonemonthwhilestoredat-80?C.Forfrozensamples,dilutionsofsamplesmustbedonerightbeforeassaying.

2.StandardCurvePreparation
WerecommendthatL-LactateStandardsbeassayedinduplicate.Astandardcurvehastoberunineachassay.
Add50µl,40µl,30µl,20µl,10µl,5µl,1µl,and0µlofL-lactateStandardtoeachwell.Thenadjustvolumeto50μl/wellwithdH2O.

3.Performtheassay

a)Add50ulofL-LactateassaysolutiontoeachwellcontainingL-Lactatestandardsandtestsamples.
b)Incubatefor30minsat37°Cincubator.Note:PleasedonotuseCO2incubator.
c)Stopthereactionbyadding50μlof0.5Maceticacidperwellfollowedbybriefgentleagitation.Note:Eliminateanyairbubblespresentinthewellsusinganeedlepriortomeasurement.
d)Measuretheabsorbanceat490nMusingamicroplatereader.

4.Calculations

a)AveragetheOD490nmvaluesofreplicatewellsofeachL-Lactatestandard,testsamples,andblank.Inordertogetthecorrectedabsorbance,subtracttheaverageOD490nmvalueoftheblank(L-LactateStandard#8)fromtheaverageOD490nmvaluesfromallstandardsandsamples.

b)MakeastandardcurvebyplottingOD490nmvaluesfromeachL-LactatestandardsasafunctionofL-Lactateconcentration.Thiscanbedonewithexcelspreadsheet.CalculatethevalueofL-Lactateinsamplesusingtheequationobtainedfromthelinearregressionofthestandardcurve.

L-Lactate(µM)=[(Correctedabsorbance)-(y-intercept)]/slope

MaterialSafetyDataSheet

Date:Oct26,2015

1.PRODUCTANDCOMPANYIDENTIFICATION

1.1Productidentifier

Productname:L-LactateAssayKitI

CatalogNo:1200011002,120001100A,120001100P,1200012002,120001200A,12001200P,1200014002,120001400A,120001400P

1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst

Forresearchuseonly.

1.3Detailsofthesupplierofthesafetydatasheet

Company:EtonBioscienceInc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4Emergencytelephonenumber

1-800-758-1630

2.HAZARDSIDENTIFICATION

2.1Classificationofthesubstanceormixture

Notahazardoussubstanceormixture

2.2GHSLabelelements,includingprecautionarystatements

Notahazardoussubstanceormixture

2.3Otherhazards

None

3.COMPOSITION/INFORMATIONONINGREDIENTS

3.1Substances

4.FIRSTAIDMEASURES

4.1Descriptionoffirstaidmeasures

Eyecontact

Checkforandremovecontactlensesandflushwithcopiousamountsofwater;assureadequateflushingbyseparatingtheeyelidswithfingers;callaphysician

SkinContact

Flushwithcopiousamountsofwater;removecontaminatedclothingandshoes;callaphysician

Inhalation

Removetofreshair.Ifnotbreathinggiveartificialrespiration;callaphysician

Ingestion

Ifswallowed,nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater;callaphysician

4.2Mostimportantsymptomsandeffects,bothacuteanddelayed

Noinformationavailable

4.3Indicationofanyimmediatemedicalattentionandspecialtreatmentneeded.

Noinformationavailable

5.FIREFIGHTINGMEASURES

5.1Extinguishingmedia

Suitableextinguishingmedia

Waterspray,alcohol-resistantfoam,drychemicalorcarbondioxide

5.2Specialhazardsarisingfromthesubstanceormixture

Noinformationavailable

5.3Adviceforfirefighters

Wearself-containedbreathingapparatusandprotectiveclothingtopreventcontactwithskinandeyes.

6.ACCIDENTALRELEASEMEASURES

6.1Personalprecautions,protectiveequipmentandemergencyprocedures

Usefullpersonalprotectiveequipment.Evacuatepersonneltosafeareas.Avoidbreathingvapors,mistorgas.

6.2Environmentalprecautions

Preventfurtherleakageorspillage.Preventproductfromenteringdrain.

6.3Methodsandmaterialforcontainmentandcleaningup

Keepinsuitable,closedcontainersfordisposal

7.HANDLINGANDSTORAGE

7.1Precautionsforsafehandling

Avoidskin/eyecontact.Useprotectiveequipmentasneeded.Washcontaminatedclothingbeforereuse.

7.2Conditionsforsafestorage,includinganyincompatibilities

Keepcontainertightlyclosed.Storeat-80?C.

7.3Specificenduse(s)

Nodataavailable.

8.EXPOSURECONTROLS/PERSONALINFORMATION

8.1ControlParameters

Thisproductcontainsnohazardousmaterialswithoccupationalexposurelimits.

8.2Exposurecontrols

Engineeringcontrols

Provideshowerandeyewashstation

Personalprotectiveequipment

	L-lactateAssaySolution
	Components		CAS#
	DMSO		67-68-5
	L-LDH		9001-60-9
	Tris-HCl,pH7.5		N/A
	Water		7732-18-5
	L-LactateAssayStandard
	Components		CAS#
	SodiumL-Lactate		867-56-1
	Tris-HCl,pH7.5		N/A
	Water		7732-18-5

EyeProtection	Wearsafetygoggles
SkinProtection	Wearprotectiveclothing
HandProtection	Wearprotectivegloves
Respiratoryprotection	WearNIOSH/MSHAapprovedrespirators

9.PHYSICALANDCHEMICALPROPERTIES

9.1Informationonbasicphysicalandchemicalproperties

L-LactateAssaySolution

Appearance	Liquid,yellowish
pH	7.5
Meltingpoint	Nodataavailable
Freezingpoint	Nodataavailable
Boilingpoint	Nodataavailable
Density	Nodataavailable
Watersolubility	Soluble

L-LactateStandard

Appearance	Liquid,colorless
pH	7.5
Meltingpoint	Nodataavailable
Freezingpoint	Nodataavailable
Boilingpoint	Nodataavailable
Density	Nodataavailable
Watersolubility	Soluble

9.2Othersafetyinformation

Nodataavailable.

10.STABILITYANDREACTIVITY

10.1Reactivity

Nodataavailable

10.2Chemicalstability

Stableunderrecommendedstorageconditions.

10.3Possibilityofhazardousreactions

Nodataavailable.

10.4Conditionstoavoid

Nodataavailable

10.5IncompatIBLematerials

Strongoxidizingagents

10.6Hazardousdecompositionproducts

Hazardousdecompositionproductsformedunderfireconditions:carbonoxides

11.TOXICOLOGICALINFORMATION

11.1Informationontoxicologicaleffects

Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.

12.ECOLOGICALINFORMATION

12.1Toxicity

Avoidreleaseintoenvironment

12.2Persistenceanddegradability

Nodataavailable

12.3.Bioaccumulativepotential

Nodataavailable

12.4Mobilityinsoil

Nodataavailable

12.5ResultsofPBTandvPvBassessment

Nodataavailable

12.6Otheradverseeffects

Nodataavailable

13.DISPOSALINFORMATION

13.1Wastetreatmentmethods

Disposeinaccordancewithlocal,state,andfederalregulations.

14.TRANSPORTINFORMATION

DOT:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

ADR/RID

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

15.REGULATORYINFORMATION

EURiskandSafetyphrases:

R36/37/38:irritatingtoeyes/respiratorysystem/skin

16.OTHERINFORMATION

Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.

Q.WhattypeofmediumshouldIuseformakingculturedcellsforLactateassay?

A.Pleaseusephenolredfreemedium.Pleasedonotusephenolredmediumsincephenolredwouldaffectabsorbancereadings.

Q.WhatenzymesisusedinLactateAssay?

A.ItisLDH.

Q.CanyoutellmethecompositionsofyourLactateAssay?

A.Unfortunatelyitisproprietary.

Q.HowdoIknowwhetherIneedtouseL-LactateorD-Lactateassaykit?

A.L-Lactateassaykitsarecommonlyusedformeasuringlactatelevelinanimalcells.

D-lactateassaykitsarecommonlyusedformeasuringlactatelevelinbacteriacells.

Q.DoIneedtomakeastandardcurveeverytime?

A.Yes,itisnecessarysinceyoucalculateyoursampleconcentrationbasedonthestandardcurve.PleasedonotusetherepresentativeLactatestandardcurveintheprotocoltocalculateconcentrationsofyoursamples.

Q.CanIusefluorescencespectroscopytomeasurereadingsforLactateassaykits?

A.No,thekitdoesnotworkwithafluorescencereadersincethekitemployscolorimetricassays.

Q.HowdoImeasureLactatefromculturedcells?

A.Youcangrowcellsinphenolred-freemedium,thenyoucanextractcellswith80%ethanol.

Q.CanIusefrozensamples?

A.Althoughitisbettertousefreshsamplesforassays,youcanusefrozensamples.Ifyouarenotusingfrozensamplesinonce,pleasemakealiquotsofyoursamplesbeforeyouputtheminafreezertopreventfromdegradingsamplesfromrepeatedfreeze-thawcycles.

Q.Mysampleformedpreciptationafteraddingaceticacid.HowcanIpreventthis?

A.Youcanstillmeasurereadingswithoutaddingaceticacid.

Q.Theprotocolrecommendsmeasuringtheabsorbanceat490nm.Is485nmcloseenough?

A.Itisokaslongasyousetuptheabsorbancebetween470and490nm.

Q.WillL-LactateassaymeasureD-Lactate?

A.No,itwont.

Q.CanIuseatransparentwall96-wellplatewithLactateKit?

A.Yes,youcan.

Q.CanEthanolinterferewiththeenzyme?

A.Theenzymecantolerateupto20%ofethanolwithoutaffectingit’senzymaticactivitywithL-Lactate.

SamVandeVelde,MeghanF.Hogan,andMarcMontminy.:mTORlinksincretinsignalingtoHIFinductioninpancreaticbetacells.PNAS,Oct2011;108:16876-16882.

JunMifune,KatrinGrage,andBerndH.A.Rehm.:ProductionofFunctionalizedBiopolyesterGranulesbyRecombinantLactococcuslactis.Appl.Envir.Microbiol.,Jul2009;75:4668-4675.

DragutinJ.Savic,WilliamM.McShan,andWilliamM.McShan.:Long-termsurvivalofStreptococcuspyogenesinrichmediaispH-dependent.Microbiology,Jun2012;158:1428-1436

KristinA.Anderson,FuminLin,ThomasJ.Ribar,RobertD.Stevens,MichaelJ.Muehlbauer,ChristopherB.Newgard,andAnthonyR.Means.:DeletionofCaMKK2fromtheLiverLowersBloodGlucoseandImprovesWhole-BodyGlucoseToleranceintheMouse.Mol.Endocrinol.,Feb2012;26:281-291.

JaninaP.Lewis,DivyaIyer,andCeciliaAnaya-Bergman.:AdaptationofPorphyromonasgingivalistomicroaerophilicconditionsinvolvesincreasedconsumptionofformateandreducedutilizationoflactate.Microbiology,Nov2009;155:3758-3774.

AmparoWolf,SameerAgnihotri,JohannMicallef,JoydeepMukherjee,NesrinSabha,RobCairns,CynthiaHawkins,andAbhijitGuha.:Hexokinase2isakeymediatorofaerobicglycolysisandpromotestumorgrowthinhumanglioblastomamultiforme.J.Exp.Med.,Feb2011;208:313-326.

InnaSerganova,AsifRizwan,XiaohuiNi,SunithaB.Thakur,JelenaVider,JamesRussell,RonaldBlasberg,andJasonA.Koutcher.:MetabolicImaging:ALinkbetweenLactateDehydrogenaseA,Lactate,andTumorPhenotype.Clin.CancerRes.,Oct2011;17:6250-6261.

ChangluLiu,JiejunWu,JessicaZhu,ChesterKuei,JingxueYu,JonathanShelton,StevenW.Sutton,XiaorongLi,SuJinYun,TaranehMirzadegan,CurtMazur,FredrikKamme,andTimothyW.Lovenberg.:METABOLISM,REGULATION,ANDSIGNALING:LactateInhibitsLipolysisinFatCellsthroughActivationofanOrphanG-protein-coupledReceptor,GPR81.J.Biol.Chem.,Jan2009;284:2811-2822.

MarinaOstroukhova,NicholasGoplen,MdZunayetKarim,LidiaMichalec,LeiGuo,QiaolingLiang,andRafeulAlam.:Theroleoflow-levellactateproductioninairwayinflammationinasthma.AmJPhysiolLungCellMolPhysiol,Feb2012;302:L300-L307.

Zhuang,Yongxian,DanielKChan,andWKeithMiskimins.“PreventingFeedbackActivationofGlycolyticATPProductionEnhancesMetforminCytotoxicityinBreastCancerCellsWhenOxidativePhosphorylationIsInhibited.”Cancer&Metabolism2.Suppl1(2014):P89.PMC.Web.6Oct.2015.

Lezi,E.etal.“EffectofExerciseonMouseLiverandBrainBioenergeticInfrastructures.”Experimentalphysiology98.1(2013):207–219.PMC.Web.6Oct.2015.

YongxianZhuang,DanielK.Chan,AllisonB.Haugrud,W.KeithMiskimins.MechanismsbyWhichLowGlucoseEnhancestheCytotoxicityofMetformintoCancerCellsBothInVitroandInVivo.September25,2014DOI:10.1371/journal.pone.0108444

BorgesFT1,MeloSA,ÖzdemirBC,KatoN,RevueltaI,MillerCA,GattoneVH2nd,LeBleuVS,KalluriR.TGF-β1-containingexosomesfrominjuredepithelialcellsactivatefibroblaststoinitiatetissueregenerativeresponsesandfibrosis.JAmSocNephrol.2013Feb;24(3):385-92.doi:10.1681/ASN.2012101031.Epub2012Dec28.

品牌介绍

Everest Biotech公司成立于2000年，由英国牛津大学一批生物化学家创建。公司科学家团队具有丰富的抗体纯化及蛋白组学专业知识，雄厚的专业背景赋予了公司能为全球科研人员提供高质量、创新性抗体产品的能力。目前，Everest Biotech可提供3000余种抗体，涉及肿瘤、自身免疫、过敏、心血管疾病、传染病、代谢、神经科学等领域。所有抗体均由Everest Biotech自主研发生产、全部经过肽亲和纯化以保证最佳的特异性和纯度、经过免疫原肽的ELISA和WB实验验证; 由PhD科学家团队提供全方位技术支持服务。Everest Biotech为众多国际知名抗体公司提供OEM服务。