Everest Biotech/Glucose Assay Kit I (5 ml)-100 Assays- Assay Solution ONLY/120003100A/(5 ml)-100 Assays- Assay Solution,Everest Biotech,,Everest Biotech,everestbiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Everest Biotech/Glucose Assay Kit I (5 ml)-100 Assays- Assay Solution ONLY/120003100A/(5 ml)-100 Assays- Assay Solution

产品编号：120003100A

市场价：¥1121.12

场地：美国(厂家直采)

产品分类：试剂盒>其它试剂盒>其它检测试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$107.80

品牌： Everest Biotech

公司：Everest Biotech

公司分类：

商品介绍

SampleTyple

Plasma,Serum,Cellculturesupernatant,otherbodyfluid

Contents

Method

Colorimetricmethodat490nm

StandardCurve

Sensitivity

8µM-400µM

ReactionTime

15minutes

Applications

ForBIOLOGicalresearch:
Glucosemeasurementinbiologicalsamples
Fordrug/pharmresearch:
DruginfluenceonGlucosemetabolism

Storage

-80°C

Notes

Materialsneededbutnotsupplied

Aplatereadercapableofmeasuringabsorbanceat490nm

AdjustablePipettesandarepeatpipettor

Distilledwater(milliQorHPLC-grade)

0.5MAceticAcid

Clearflatbottom96-wellplatesifnotincludedinthekitpurchased

Shipping

Icepacks

ReagentPreparation

Note:Allreagentsarefrozen.Werecommendyouspinthestandardvialbeforeopening.

1.GlucoseStandard(2x)

Note:Pleasedon’tdodilutionintheprovidedGlucosestandardvialitself.

Thevialcontains500µlof800µMGlucoseStandard.Thestandardmustbeequilibratedtoroomtemperaturebeforeuse.Dilute500µlof800µMGlucoseStandardwith500µlofdH2Otopreparea400μMGlucoseStandard.1mlofdilutedstandardisenoughformaking3standardcurvesifassayedinduplicate.Storeat-80?C.

2.GlucoseAssaySolution

Thesolutioncontainsenzymesthatarelightsensitive.Thesolutionmustbethawedonicebeforeuse.Besttoaliquottheamountneededanduseitalltopreventthawing/freezingcycles.Freezeandstorealiquotsat-80oC

Protocol

1.SamplePreparation

Serum/Plasma/otherbodyfluid/cellculturesupernatant
Serum,Plasma,otherbodyfluid,orcellculturesupernatantcanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereADIngsarewithinthestandardcurve.YoursamplescanbedilutedwithdH2O.

SolidSamples
Solidsamples,suchastissues,canbefirsthomogenizedandextractedwithethanol(80%)withatissues/Ethanolratioof1:8(1hrat4°C)followedbycentrifugationat10,000xg.Theclearsupernatantsthencanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;.…..)toensurethereadingsarewithinthestandardcurve.YoursamplescanbedilutedwithdH2O.

AddingSamples
Add50μlofsamplestoeachwell.
Werecommendthatsamplesbeassayedinduplicate.

Note:
Ifpreparedsamplesarenotassayedthesameday,storethesamplesat-80?C.Thesampleswillbestableforonemonthwhilestoredat-80?C.Forfrozensamples,dilutionsofsamplesmustbedonerightbeforeassaying.

2.StandardCurvePrepation

WerecommendthatGlucoseStandardsbeassayedinduplicate.Astandardcurvehastoberunineachassay.
Add50µl,40µl,30µl,20µl,10µl,5µl,1µl,and0µlofdilutedGlucoseStandardtoeachwell.Thenadjustvolumeto50μl/wellwithdH2O.

3.Performtheassay
a.Add50µlofGlucoseAssaySolutiontoeachwellcontainingtheGlucoseStandardsandtestsamples.

b.Incubatefor15minsat37°Cincubator.Note:PleasedonotuseCO2incubator.

c.Stopthereactionbyadding50μlof0.5Maceticacidperwellfollowedbybriefgentleagitation.Note:Eliminateanyairbubblespresentinthewellsusinganeedlepriortomeasurement.

d.Measuretheabsorbanceat490nmusingamicroplatereader.

4.Calculation

a.AveragetheOD490nmvaluesofreplicatewellsofeachGlucosestandard,testsamples,andblank.Inordertogetthecorrectedabsorbance,subtracttheaverageOD490nmvalueoftheblankfromtheaveragedOD490nmvaluesfromallstandardsandsamples.

b.MakeastandardcurvebyplottingOD490nmvaluesfromeachglucosestandardsasafunctionofGlucoseconcentration.Thiscanbedonewithexcelspreadsheet.CalculatetheconcentrationofGlucoseinsamplesusingtheequationobtainedfromthelinearregressionofthestandardcurve.

Glucose(μM)=[(Correctedabsorbance)-(y-intercept)]/Slope

MaterialSafetyDataSheet

Date:Oct26,2015

1.PRODUCTANDCOMPANYIDENTIFICATION

1.1Productidentifier

Productname:GlucoseAssayKitI

CatalogNo:1200031002,120003100A,120003100P,1200032002,120003200A,12003200P,1200034002,120003400A,120003400P

1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst

Forresearchuseonly.

1.3Detailsofthesupplierofthesafetydatasheet

Company:EtonBioscienceInc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4Emergencytelephonenumber

1-800-758-1630

2.HAZARDSIDENTIFICATION

2.1Classificationofthesubstanceormixture

Notahazardoussubstanceormixture

2.2GHSLabelelements,includingprecautionarystatements

Notahazardoussubstanceormixture

2.3Otherhazards

None

3.COMPOSITION/INFORMATIONONINGREDIENTS

3.1Substances

4.FIRSTAIDMEASURES

4.1Descriptionoffirstaidmeasures

Eyecontact

Checkforandremovecontactlensesandflushwithcopiousamountsofwater;assureadequateflushingbyseparatingtheeyelidswithfingers;callaphysician

SkinContact

Flushwithcopiousamountsofwater;removecontaminatedclothingandshoes;callaphysician

Inhalation

Removetofreshair.Ifnotbreathinggiveartificialrespiration

Ingestion

Ifswallowed,nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater.

4.2Mostimportantsymptomsandeffects,bothacuteanddelayed

Noinformationavailable

4.3Indicationofanyimmediatemedicalattentionandspecialtreatmentneeded.

Noinformationavailable

5.FIREFIGHTINGMEASURES

5.1Extinguishingmedia

Suitableextinguishingmedia

Waterspray,alcohol-resistantfoam,drychemicalorcarbondioxide

5.2Specialhazardsarisingfromthesubstanceormixture

Noinformationavailable

5.3Adviceforfirefighters

Wearself-containedbreathingapparatusandprotectiveclothingtopreventcontactwithskinandeyes.

6.ACCIDENTALRELEASEMEASURES

6.1Personalprecautions,protectiveequipmentandemergencyprocedures

Usefullpersonalprotectiveequipment.Evacuatepersonneltosafeareas.Avoidbreathingvapors,mistorgas.

6.2Environmentalprecautions

Preventfurtherleakageorspillage.Preventproductfromenteringdrain.

6.3Methodsandmaterialforcontainmentandcleaningup

Keepinsuitable,closedcontainersfordisposal

7.HANDLINGANDSTORAGE

7.1Precautionsforsafehandling

Avoidskin/eyecontact.Useprotectiveequipmentasneeded.Washcontaminatedclothingbeforereuse.

7.2Conditionsforsafestorage,includinganyincompatibilities

Keepcontainertightlyclosed.Storeat-80?C.

7.3Specificenduse(s)

Nodataavailable.

8.EXPOSURECONTROLS/PERSONALINFORMATION

8.1ControlParameters

Thisproductcontainsnohazardousmaterialswithoccupationalexposurelimits.

8.2Exposurecontrols

Engineeringcontrols

Provideshowerandeyewashstation

Personalprotectiveequipment

	GlucoseAssaySolution
	Components		CAS#
	Potassiumphosphatemonobasic		7778-77-0
	Potassiumphosphatedibasic		7758-11-4
	GlucoseOxidase		9001-37-0
	GlucoseAssayStandard
	Components		CAS#
	SodiumD-Glucose		50-99-7
	Potassiumphosphatemonobasic		7778-77-0
	Potassiumphosphatedibasic		7758-11-4
	Water		7732-18-5

EyeProtection	Wearsafetygoggles
SkinProtection	Wearprotectiveclothing
HandProtection	Wearprotectivegloves
Respiratoryprotection	WearNIOSH/MSHAapprovedrespirators

9.PHYSICALANDCHEMICALPROPERTIES

9.1Informationonbasicphysicalandchemicalproperties

Appearance	Clear,colorlessliquid
pH	7.0
Meltingpoint	Nodataavailable
Freezingpoint	Nodataavailable
Boilingpoint	Nodataavailable
Density	Nodataavailable
Watersolubility	Soluble

9.2Othersafetyinformation

Nodataavailable.

10.STABILITYANDREACTIVITY

10.1Reactivity

Nodataavailable

10.2Chemicalstability

Stableunderrecommendedstorageconditions.

10.3Possibilityofhazardousreactions

Nodataavailable.

10.4Conditionstoavoid

Nodataavailable

10.5IncompatIBLematerials

Strongoxidizingagents

10.6Hazardousdecompositionproducts

Hazardousdecompositionproductsformedunderfireconditions:carbonoxides

11.TOXICOLOGICALINFORMATION

11.1Informationontoxicologicaleffects

Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.

12.ECOLOGICALINFORMATION

12.1Toxicity

Avoidreleaseintoenvironment

12.2Persistenceanddegradability

Nodataavailable

12.3.Bioaccumulativepotential

Nodataavailable

12.4Mobilityinsoil

Nodataavailable

12.5ResultsofPBTandvPvBassessment

Nodataavailable

12.6Otheradverseeffects

Nodataavailable

13.DISPOSALINFORMATION

13.1Wastetreatmentmethods

Disposeinaccordancewithlocal,state,andfederalregulations.

14.TRANSPORTINFORMATION

DOT:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

ADR/RID

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

15.REGULATORYINFORMATION

EURiskandSafetyphrases:

R36/37/38:irritatingtoeyes/respiratorysystem/skin

16.OTHERINFORMATION

Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.

ZakikhaniM,BazileM,HashemiS,JaveshghaniS,AvizonisD,etal.(2012)AlterationsinCellularEnergyMetabolismAssociatedwiththeAntiproliferativeEffectsoftheATMInhibitorKU-55933andwithMetformin.PLoSONE7(11):e49513.doi:10.1371/journal.pone.0049513

ShuangLiang,*PaolaGalluzzo,*AnnaSobol,SylviaSkucha,BrittanyRambo,andMaurizioBocchetta.MultimodalityApproachestoTreatHypoxicNon–SmallCellLungCancer(NSCLC)Microenvironment.GenesCancer.2012February;3(2):141–151.

ZHANG,YANBINetal.GlucoseTransporter3PerformsaCriticalRoleinmTOR-MediatedOncogenicGlycolysisandTumOrigenesis.OncologyLetters9.6(2015):2809–2814.PMC.Web.6Oct.2015.

EyalAmiel,BartEverts,ToriC.Freitas,IrahL.King,JonathanD.Curtis,ErikaL.PearceandEdwardJ.Pearce.InhibitionofMechaNISTicTargetofRapamycinPromotesDendriticCellActivationandEnhancesTherapeuticAutologousVaccinationinMice.JImmunol2012;189:2151-2158

ZhiguoLi,JieLi,PenpengBi,YingLu,GrantBurcham,BennetD.Elzey,TimothyRatliff,StephenF.Konieczny,NihalAhmad,ShihuanKuang,andXiaoquiLiu.Plk1PhosphorylationofPTENcausesatumor-PromotingMetabolicState.Mol.Cell.Biol.October2014vol.34no.193642-3661

QingsongCai,TongLin,SushamaKamarajugadda,andJianrongLu.RegulationofGlycolysisandtheWarburgEffectbyEstrogen-relatedReceptors.Oncogene.2013April18;32(16):2079–2086.

WhattypeofmediumshouldIuseformakingculturedcellsforthisassay?

Pleaseusephenolredfreemedium.Pleasedonotusephenolredmediumsincephenolredwouldaffectabsorbancereadings.

Whatenzymeisusedinthisassay?

ItisGlucoseOxidase.

Whatarethecompositionsofthisassay?

Unfortunatelyitisproprietary.

Whatisthesensitivityofthisassay?

Itis8μM-400μM.

DoIneedtomakeastandardcurveeverytime?

Yesitisnecessarysinceyoucalculateyoursampleconcentrationbasedonthestandardcurve.Pleasedonotusetherepresentativestandardcurveintheprotocoltocalculateconcentrationsofyoursamples.

HowmuchsampledoIneedforthisassay?

End-userswouldhavetoperformanexperimenttodetermineoptimalvolumeofsampleswhosereadingsfallwithinthestandardcurverange.Pleaserefertopublicationswithwhichthisproductwasusedtoseehowmuchsampleshavebeenusedinsimilarexperiments.

HowcanIdilutemysamples?

YoursamplesmaybedilutedwithPBSordH2O.

CanIusefluorescencespectroscopytomeasurereadingsforthisassay?

No,thekitdoesnotworkwithafluorescencereadersincethekitemployscolorimetricassays.

CanIusefrozensamples?

Althoughitisbettertousefreshsamplesforassays,youcanusefrozensamples.Ifyouarenotusingfrozensamplesinonce,pleasemakealiquotsofyoursamplesbeforeyouputtheminafreezertopreventfromdegradingsamplesfromrepeatedfreeze-thawcycles.

Mysampleformedprecipitationafteraddingaceticacid.HowcanIpreventthis?

Youcanstillmeasurereadingswithoutaddingaceticacid.

Theprotocolrecommendsmeasuringtheabsorbanceat490nm.Is485nmcloseenough?

Itisokaslongasyousetuptheabsorbancebetween470and490nm.

Whyaremystandardcurvevaluesdifferentthantheoneshownonthedatasheet?

Manyfactorsinfluencethevaluessuchasroomtemperature,incubationtime,handling,etc.

Aretrialkitsavailable?

Yestheyareavailableforfirsttimecustomers;however,youwillberesponsibleforshippingandhandlingfee.Pleasecontact

formoredetails.

品牌介绍

Everest Biotech公司成立于2000年，由英国牛津大学一批生物化学家创建。公司科学家团队具有丰富的抗体纯化及蛋白组学专业知识，雄厚的专业背景赋予了公司能为全球科研人员提供高质量、创新性抗体产品的能力。目前，Everest Biotech可提供3000余种抗体，涉及肿瘤、自身免疫、过敏、心血管疾病、传染病、代谢、神经科学等领域。所有抗体均由Everest Biotech自主研发生产、全部经过肽亲和纯化以保证最佳的特异性和纯度、经过免疫原肽的ELISA和WB实验验证; 由PhD科学家团队提供全方位技术支持服务。Everest Biotech为众多国际知名抗体公司提供OEM服务。