Everest Biotech/TurboNuclease 100,000 Units/1400010100/100,000 Units,Everest Biotech,,Everest Biotech,everestbiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Everest Biotech/TurboNuclease 100,000 Units/1400010100/100,000 Units

产品编号：1400010100

市场价：¥4524.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>其他>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$435.00

品牌： Everest Biotech

公司：Everest Biotech

公司分类：

商品介绍

ProteinType:
Endonuclease

Source:
E.coli

UnitDefinition:
OneunitofTurboNucleaseconverts1.0OD₂₆₀ofsalmonspermDNAintoacid-solublenucleotidesin30minutesat37^oCinareactionbufferof50mMTris-HCl,pH8.0and1mMMgCl₂.Thiscorrespondstocompletedigestionof50µgofsalmonspermDNAintooligonucleotides.TurboNucleasehassimilarspecificactivityasBenzonase

Storage:
StoreTurboNucleaseat-20^oC.

EndotoxinLevel:
<0.1EU/1,000unitsofTurboNucleaseasdeterminedbytheEndo-SafeLALAssay.

Formulation:

250units/µlin50mMTris-HCl,pH8.0,50mMNaCl,5mMMgCl₂and50%Glycerol

TurboNucleaseActivityandPurity:

	50µgofsalmonspermDNAwasincubatedwiththeindicatedunitsofTurboNucleaseandanotherbrandofnucleaseat37^oCfor30minutesinabufferof50mMTris-HCl,pH8.0and1mMMgCl₂.DNAdigestionwasmonitoredbyagarosegel.
	TurboNucleaseispurifiedthroughaproprietaryprocessthatachievespurityof>99%.TurboNucleasehasnodetectableproteaseactivity.

TurboNucleasecanbeusedto

reduceviscosityofcelllysateandreducebackpressureofcolumnloADIng

removenucleicacidcontaminationfromsamplepreparations,reducenucleicacidcontaminationofNicolumnpurification

reducesmearinginSDS-PAGEwhenusedwith10%SDSorGelLoadingDyetomakewholecelllysate

reduceorpreventclumpingofconcentratedcellsandthawedcells

replacescrudeDNaseIinmanyapplications

Toreduceviscosityofcelllysate,10-500unitsofTurboNucleasecanbeusedforeachgramofcellpaste.Generally,addingTurboNucleasetocelllysateat25U/mlissufficienttoreducelysateviscosity.

Theefficiencyofviscosityreductionmayvarywithbuffers,celltypes,andcelllysismethodsused.Duetoitshighspecificactivity,thetotalamountTurboNucleaseaddedislessthan0.1ug/mloflysateandwillnotcomplicateanydownstreamprocess.

LareScaleCellLysis

MakefreshcoldLysisBuffer
LysisBuffershouldbeabufferinwhichthetargetproteinissoluble.
TheLysisBuffershouldbecompatIBLewithdownstreampurificationprocesses,e.g.minimalamountofEDTAorDTTifNicolumnwillbeused.

HereisanexampleofLysisBuffer
25mMTris-HCl,pH8.0
500mMNaCl
14mMbeta-mercaptoethanol
Detergentcanbeincludedforlesssolubleproteinsorwhenproteinsolubilityisunknown.1%TritonX-100hasnoeffectonTurboNucleaseactivity.

TurboNucleasehasthesameactivityin150mMNaClor500mMNaCland400mMimidazole.

ResUSPendthawedcellpasteinLysisBuffer
Use2-10mlLysisBufferforeachgramofcellpaste.TurboNucleasecanreducetheamountofLysisBufferused.
Weroutinelyuse2mloflysisbufferforeachgramofcellpellets.
AddTurboNucleaseto25unit/ml
Proteaseinhibitorscanbeaddedatthesametime.
IfthelysisbuffercontainsEDTAorEGTA,add10-foldmoreTurboNuclease.
Lyzecellsbymechanicalorchemicalmethodsoniceoratroomtemperature
TurboNucleasealsoreducestheviscosityoflysatelyzedbymicrofluidizer.
Clearlysatebycentrifugationforcolumnloading
Thereducedviscositymakesitpossibletoclearthelysateatlowerspeed.35,000g(~16,000rpm)for1hourissufficient.
Lysatecanbeloadedto"Crude"columnswithoutclearance.

ParallelLysisofMultipleInsectCellSamples

Freezecellspelletsof5-10mlcultureondryicebriefly
Freezeandthawfacilitateslysis.
Thawthefrozenpelletsandcompletelyresuspendin~1mlLysisBufferwithTurboNuclease
Transferthecellsuspensiontoamicrotubeandsitthetubesonafloaterrack
LyzecellsusinganUltrasonicCleanerwithicewaterbathfor10min
UltrasonicCleaner(manychemistsuseit)ismuchcheaperthanprobesonicator.ItcostsafewhundredsUSdollarsforanewmodelandlessthan$100forausedoneorajewelrycleanerfromaconsumergoodsstore.UltrasonicCleanerismuchbetterandcheaperthanthosefancymulti-probesonicatorswiththefollowingadvantages.

Thereisnocross-contaminationsinceeachsampleisenclosedinamicrotube.

Thesamplesarealwayscoldaslongasiceisaddedinthewater-bath.

Thereisnolimitonthenumberofsamplesprocessedinparallel.AsmallUltrasonicCleanercaneasilyhold48samples

Thelysatecanbeusedforanalysesofproteinexpressionofwholecelllysate,solublelysate,oraffinitypull-down.

EtonBioLysisBuffer
25mMTris-HCl,pH8.0
500mMNaCl
20mMImidazole,pH8.0
14mMbeta-mercaptoethanol
0.5%TritonX-100
25units/mlTurboNuclease

MaterialSafetyDataSheet

Date:Oct26,2015

1.PRODUCTANDCOMPANYIDENTIFICATION

1.1Productidentifier

Productname:TurboNuclease

CatalogNo:1400010050,1400010100,1400010250

1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst

Forresearchuseonly.

1.3Detailsofthesupplierofthesafetydatasheet

Company:EtonBioscienceInc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4Emergencytelephonenumber

1-800-758-1630

2.HAZARDSIDENTIFICATION

2.1Classificationofthesubstanceormixture

Notahazardoussubstanceormixture

2.2GHSLabelelements,includingprecautionarystatements

Notahazardoussubstanceormixture

2.3Otherhazards

None

3.COMPOSITION/INFORMATIONONINGREDIENTS

3.1Substances

Synonyms:RecombinantSerratiamarcescensextracellularendonuclease

CAS-No	EC-No.	Index-No	Classification	Concentration
Water
7732-18-5	231-791-2	-	-	>=45%
Glycerol
56-81-5	200-289-5	-	-	50%
Tris(hydroxymethyl)aminomethanefreebase
77-86-1	201-064-4	-	Xi,R36/37/38	0.6%
Enzyme
-	-	-	-	<=0.025%
Magnesiumchloridehexahydrate
7791-18-6	232-094-6	-	-	0.1%
Sodiumchloride
7647-14-5	231-598-3	-	-	0.3%

ForthefulltextoftheR-phrasesmentionedinthisSection,seeSection16.

4.FIRSTAIDMEASURES

4.1Descriptionoffirstaidmeasures

Eyecontact

Checkforandremovecontactlensesandflushwithcopiousamountsofwater;assureadequateflushingbyseparatingtheeyelidswithfingers;callaphysician

SkinContact

Flushwithcopiousamountsofwater;removecontaminatedclothingandshoes;callaphysician

Inhalation

Removetofreshair.Ifnotbreathinggiveartificialrespiration

Ingestion

Ifswallowed,nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater.

4.2Mostimportantsymptomsandeffects,bothacuteanddelayed

Noinformationavailable

4.3Indicationofanyimmediatemedicalattentionandspecialtreatmentneeded.

Noinformationavailable

5.FIREFIGHTINGMEASURES

5.1Extinguishingmedia

Suitableextinguishingmedia

Waterspray,alcohol-resistantfoam,drychemicalorcarbondioxide

5.2Specialhazardsarisingfromthesubstanceormixture

Noinformationavailable

5.3Adviceforfirefighters

Wearself-containedbreathingapparatusandprotectiveclothingtopreventcontactwithskinandeyes.

6.ACCIDENTALRELEASEMEASURES

6.1Personalprecautions,protectiveequipmentandemergencyprocedures

Usefullpersonalprotectiveequipment.Evacuatepersonneltosafeareas.Avoidbreathingvapors,mistorgas.

6.2Environmentalprecautions

Preventfurtherleakageorspillage.Preventproductfromenteringdrain.

6.3Methodsandmaterialforcontainmentandcleaningup

Keepinsuitable,closedcontainersfordisposal

7.HANDLINGANDSTORAGE

7.1Precautionsforsafehandling

Avoidskin/eyecontact.Useprotectiveequipmentasneeded.Washcontaminatedclothingbeforereuse.

7.2Conditionsforsafestorage,includinganyincompatibilities

Keepcontainertightlyclosed.Storeat-80?C.

7.3Specificenduse(s)

Nodataavailable.

8.EXPOSURECONTROLS/PERSONALINFORMATION

8.1ControlParameters

Thisproductcontainsnohazardousmaterialswithoccupationalexposurelimits.

8.2Exposurecontrols

Engineeringcontrols

Provideshowerandeyewashstation

Personalprotectiveequipment

EyeProtection	Wearsafetygoggles
SkinProtection	Wearprotectiveclothing
HandProtection	Wearprotectivegloves
Respiratoryprotection	WearNIOSH/MSHAapprovedrespirators

9.PHYSICALANDCHEMICALPROPERTIES

9.1Informationonbasicphysicalandchemicalproperties

Appearance	Clear,colorlessliquid
pH	8.0
Meltingpoint	Nodataavailable
Flashpoint	Nodataavailable
Boilingpoint	Nodataavailable
Ignitiontemperature	Nodataavailable
Lowerexplosionlimit	Nodataavailable
Upperexplosionlimit	Nodataavailable
Watersolubility	Soluble

9.2Othersafetyinformation

Nodataavailable.

10.STABILITYANDREACTIVITY

10.1Reactivity

Nodataavailable

10.2Chemicalstability

Stableunderrecommendedstorageconditions.

10.3Possibilityofhazardousreactions

Nodataavailable.

10.4Conditionstoavoid

Nodataavailable

10.5Incompatiblematerials

Strongoxidizingagents

10.6Hazardousdecompositionproducts

Hazardousdecompositionproductsformedunderfireconditions:carbonoxides

11.TOXICOLOGICALINFORMATION

11.1Informationontoxicologicaleffects

Acutetoxicity

Nodataavailable

Irritationandcorrosion

Nodataavailable

Sensitisation

Prolongedorrepeatedexposuremaycauseallergicreactionsincertainsensitiveindividuals

Chronicexposure

IARC:Nocomponentofthisproductpresentatlevelsgreaterthanorequalto0.1%isidentifiedasprobable,possibleorconfirmedhumancarcinogenbyIARC.

SignsandSymptomsoExposure

Prolongedorrepeatedexposurecancause:Nausea,Headache,Vomiting

PotentialHealthEffects

Inhalation	Maybeharmfulifinhaled.Maycauserespiratorytractirritation
Skin	Maybeharmfulifabsorbedthroughskin.Maycauseskinirritation
Eyes	Maycauseeyeirritation
Ingestion	Maybeharmfulifswallowed.
Targetorgans	Kidney

12.ECOLOGICALINFORMATION

12.1Toxicity

Avoidreleaseintoenvironment

12.2Persistenceanddegradability

Nodataavailable

12.3.Bioaccumulativepotential

Nodataavailable

12.4Mobilityinsoil

Nodataavailable

12.5ResultsofPBTandvPvBassessment

Nodataavailable

12.6Otheradverseeffects

品牌介绍

Everest Biotech公司成立于2000年，由英国牛津大学一批生物化学家创建。公司科学家团队具有丰富的抗体纯化及蛋白组学专业知识，雄厚的专业背景赋予了公司能为全球科研人员提供高质量、创新性抗体产品的能力。目前，Everest Biotech可提供3000余种抗体，涉及肿瘤、自身免疫、过敏、心血管疾病、传染病、代谢、神经科学等领域。所有抗体均由Everest Biotech自主研发生产、全部经过肽亲和纯化以保证最佳的特异性和纯度、经过免疫原肽的ELISA和WB实验验证; 由PhD科学家团队提供全方位技术支持服务。Everest Biotech为众多国际知名抗体公司提供OEM服务。